Rat Total MMP-9 ELISA Kit

ELISA Kits

$350.00 – $450.00

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Datasheet

SDS File

TARGETS	MMP9
SPECIES	Rat
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL(diluent)
Sensitivity	13.88 pg/mL
Range	78.13 pg/mL – 5000 pg/mL
Assay Time	3.5 h
Recovery	86% – 114%
Average Recovery	1.03

Sample Type	Serum, plasma and cell culture supernatant
Sample Volume	100 μL(diluent)
Sensitivity	37.135 pg/mL
Range	78.13 pg/mL – 5000 pg/mL
Assay Time	3.5 h
Recovery	89% – 114%
Average Recovery	0.98
Intra Precision	7.7% – 9.5%
Inter-Precision	5.6% – 8.1%
Platform	ELISA
Plate	Detachable 96-well plate
Size	96T/48T
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-MMP-9 monoclonal antibody Rat MMP-9 freeze-dried standard MMP-9 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-rat MMP-9 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, MMP-9 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of MMP-9 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Targets

MMP9

MMP9 Target Infomation Overview

Target Symbol: MMP9, matrix metallopeptidase 9
Gene Groups: M10 matrix metallopeptidases
Previous Names: CLG4B
Alias Names: matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)

MMP9, matrix metallopeptidase 9 Target Infomation by Species

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Human MMP9 Target Information

Target Symbol: MMP9, matrix metallopeptidase 9
Alias:
- 92 kDa gelatinase
- 92 kDa type IV collagenase
- CLG4B
- GELB
- macrophage gelatinase
- MANDP2
- matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
- matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
- matrix metalloproteinase-9
- MMP-9
- type V collagenase
NCBI_Gene: 4318
UniProtKB: P14780

Human MMP9 Predicted Functions

Enables identical protein binding activity; metalloendopeptidase activity; and serine-type endopeptidase activity. Involved in several processes, including cellular response to UV-A; negative regulation of apoptotic signaling pathway; and positive regulation of keratinocyte migration. Located in extracellular space. Implicated in several diseases, including Down syndrome; anodontia; artery disease (multiple); autoimmune disease (multiple); and chronic obstructive pulmonary disease (multiple). Biomarker of several diseases, including artery disease (multiple); autoimmune disease (multiple); brain disease (multiple); carcinoma (multiple); and lung disease (multiple).

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Mouse Mmp9 Target Information

Target Symbol: Mmp9, matrix metallopeptidase 9
Alias:
- AW743869
- B/MMP9
- Clg4b
- collagenase IVB, basement membrane, 92 kDa
- expressed sequence AW743869
- Gel B
- Gelatinase B
- gelatinase B
- MMP-9
NCBI_Gene: 17395

Mouse Mmp9 Predicted Functions

Enables metalloendopeptidase activity. Involved in negative regulation of epithelial cell differentiation involved in kidney development and response to amyloid-beta. Acts upstream of or within several processes, including collagen catabolic process; embryo implantation; and extracellular matrix organization. Located in collagen-containing extracellular matrix. Is expressed in several structures, including alimentary system; central nervous system; early conceptus; limb mesenchyme; and skeleton. Human ortholog(s) of this gene implicated in several diseases, including Down syndrome; anodontia; artery disease (multiple); autoimmune disease (multiple); and lung disease (multiple). Orthologous to human MMP9 (matrix metallopeptidase 9).

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Rat Mmp9 Target Information

Target Symbol: Mmp9, matrix metallopeptidase 9
Alias:
- 92 kDa gelatinase
- 92 kDa type IV collagenase
- 92-kDa type IV collagenase
- gelatinase B
- GELB
- matrix metalloproteinase 9 (gelatinase B 92-kDa type IV collagenase)
- matrix metalloproteinase 9 (gelatinase B, 92-kDa type IV collagenase)
- matrix metalloproteinase-9
- MMP-9
NCBI_Gene: 81687

Rat Mmp9 Predicted Functions

Enables fibronectin binding activity and metallopeptidase activity. Involved in several processes, including cellular response to cell-matrix adhesion; cellular response to cytokine stimulus; and cellular response to iron(III) ion. Located in extracellular space. Part of protein-containing complex. Used to study artery disease (multiple); brain ischemia (multiple); congestive heart failure; glomerulosclerosis; and lung disease (multiple). Biomarker of several diseases, including artery disease (multiple); brain disease (multiple); chronic obstructive pulmonary disease (multiple); glucose metabolism disease (multiple); and kidney disease (multiple). Human ortholog(s) of this gene implicated in several diseases, including Down syndrome; anodontia; artery disease (multiple); autoimmune disease (multiple); and chronic obstructive pulmonary disease (multiple). Orthologous to human MMP9 (matrix metallopeptidase 9).

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