Rat CCL3/MIP-1α ELISA Kit

ELISA Kits

$350.00 – $450.00

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Datasheet

SDS File

TARGETS	CCL3
SPECIES	Rat
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	80 μL 1×Assay Buffer and 20 μL sample
Sensitivity	0.09 pg/mL
Range	6.25 pg/mL – 400 pg/mL
Assay Time	3.5 h
Recovery	90% – 119%
Average Recovery	1.05

Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	80 μL 1×Assay Buffer and 20 μL sample
Sensitivity	0.09 pg/mL
Range	6.25 pg/mL – 400 pg/mL
Assay Time	3.5 h
Recovery	90% – 119%
Average Recovery	1.05
Intra Precision	4.8% – 5.0%
Inter-Precision	3.5%-4.6%
Platform	ELISA
Plate	Detachable 96-well plate
Size	96T/48T
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-CCL3/MIP-1α monoclonal antibody Rat CCL3/MIP-1α freeze-dried standard CCL3/MIP-1α detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-rat CCL3 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, CCL3 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of CCL3 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Targets

CCL3

CCL3 Target Infomation Overview

Target Symbol: CCL3, C-C motif chemokine ligand 3
Gene Groups: Chemokine ligands
Alias: G0S19-1; LD78ALPHA; MIP-1-alpha
Previous Names: SCYA3
Alias Names: small inducible cytokine A3 (homologous to mouse Mip-1a); chemokine (C-C motif) ligand 3

CCL3, C-C motif chemokine ligand 3 Target Infomation by Species

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Human CCL3 Target Information

Target Symbol: CCL3, C-C motif chemokine ligand 3
Alias:
- C-C motif chemokine 3
- chemokine (C-C motif) ligand 3
- G0/G1 switch regulatory protein 19-1
- G0S19-1
- LD78 alpha beta
- LD78ALPHA
- macrophage inflammatory protein 1-alpha
- MIP-1-alpha
- MIP1A
- PAT 464.1
- SCYA3
- SIS-beta
- small inducible cytokine A3 (homologous to mouse Mip-1a)
- small-inducible cytokine A3
- tonsillar lymphocyte LD78 alpha protein
NCBI_Gene: 6348
UniProtKB: P10147

Human CCL3 Predicted Functions

Enables several functions, including calcium-dependent protein kinase C activity; chemoattractant activity; and chemokine receptor binding activity. Involved in several processes, including cellular response to cytokine stimulus; leukocyte chemotaxis; and positive regulation of intracellular signal transduction. Located in cytosol and extracellular space. Implicated in human immunodeficiency virus infectious disease. Biomarker of COVID-19; alcoholic hepatitis; glomerulonephritis (multiple); kidney failure (multiple); and systemic scleroderma.

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Mouse Ccl3 Target Information

Target Symbol: Ccl3, chemokine (C-C motif) ligand 3
Alias:
- AI323804
- CCL3
- expressed sequence AI323804
- G0S19-1
- LD78alpha
- macrophage inflammatory protein-1 alpha
- macrophage inflammatory protein-1alpha
- MIP-1 alpha
- MIP-1alpha
- MIP1-(a)
- MIP1-alpha
- Mip1a
- Scya3
- small inducible cytokine A3
NCBI_Gene: 20302

Mouse Ccl3 Predicted Functions

Enables chemokine activity. Involved in several processes, including astrocyte cell migration; macrophage chemotaxis; and positive regulation of macromolecule metabolic process. Located in extracellular space. Is expressed in articular cartilage; cartilage; knee joint; lung; and radius. Human ortholog(s) of this gene implicated in human immunodeficiency virus infectious disease. Orthologous to several human genes including CCL3 (C-C motif chemokine ligand 3).

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Rat Ccl3 Target Information

Target Symbol: Ccl3, C-C motif chemokine ligand 3
Alias:
- C-C motif chemokine 3
- chemokine (C-C motif) ligand 3
- macrophage inflammatory protein 1 alpha (Small inducible cytokine A3)
- macrophage inflammatory protein 1-alpha
- MIP-1-alpha
- MIP-1a
- Scya3
- small inducible cytokine A3
- small-inducible cytokine A3
NCBI_Gene: 25542
UniProtKB: P50229

Rat Ccl3 Predicted Functions

Enables chemokine activity. Involved in several processes, including neutrophil chemotaxis; positive regulation of cytosolic calcium ion concentration; and positive regulation of osteoclast differentiation. Predicted to be located in cytosol and extracellular region. Predicted to be active in extracellular space. Biomarker of several diseases, including Chagas disease; anterior uveitis; nephritis (multiple); renal fibrosis; and status epilepticus. Human ortholog(s) of this gene implicated in human immunodeficiency virus infectious disease. Orthologous to several human genes including CCL3 (C-C motif chemokine ligand 3).

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