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EasyGo!™ Mouse IL-6 One-Step ELISA Kit
$400.00 – $500.00
Sample Type | Cell culture supernates, serum and plasma |
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Sample Volume | Serum or plasma: 10μL; cell culture supernatant: 50μL |
Sensitivity | 4.92 pg/mL |
Range | 7.81 pg/mL – 500 pg/mL |
Assay Time | 1.5 h |
Recovery | 89% – 112% |
Average Recovery | 1.02 |
Intra Precision | 2.3% – 3.0% |
Inter-Precision | 2.1% – 5.3% |
Platform | ELISA |
Plate | Detachable 96-well plate |
Size | 96T/48T |
Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
Delivery | 4℃ blue ice transportation |
Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-6 monoclonal antibody Mouse IL-6 freeze-dried standard IL-6 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
Assay Principle | This assay employs the quantitative sandwich enzyme immunoassay technique. Amonoclonal antibody specific for Mouse IL-6 has been immobilized onto microwells, and two pellets of the biotin-linked detect antibody specific for IL-6 (light yellow) and streptavidin-HRP (purple) are pre-placed in the microwells, sealed by the adhesive film. Standard or samples are pipetted intothe wells, then IL-6 present is bound by the immobilized antibody and detect antibody, of whichthe latter is conjugated with streptavidin-HRP in the incubation. After washing, substrate solutionreacts with HRP and color develops in proportion to the amount of IL-6 bound by the immobilizedantibody. The color development is stopped and the intensity of the color is measured bymicroplate reader. |
Targets
IL6
IL6 Target Infomation Overview
- Target Symbol: IL6, interleukin 6
- Gene Groups: Interferons; Interleukins; Interleukin 6 type cytokine family
- Alias: IL-6; BSF2; HGF; HSF
- Previous Names: IFNB2
- Alias Names: interferon, beta 2; interleukin 6 (interferon, beta 2)
IL6, interleukin 6 Target Infomation by Species
Human IL6 Target Information
- Target Symbol: IL6, interleukin 6
- Alias:
- B cell stimulatory factor-2
- B-cell differentiation factor
- B-cell stimulatory factor 2
- BSF-2
- BSF2
- CDF
- CTL differentiation factor
- HGF
- HSF
- hybridoma growth factor
- IFN-beta-2
- IFNB2
- IL-6
- interferon beta-2
- interferon, beta 2
- interleukin BSF-2
- interleukin-6
- NCBI_Gene: 3569
- UniProtKB: P05231
Human IL6 Predicted Functions
Enables cytokine activity and growth factor activity. Contributes to interleukin-6 receptor binding activity. Involved in several processes, including positive regulation of cell population proliferation; positive regulation of macromolecule metabolic process; and regulation of peptide hormone secretion. Acts upstream of or within several processes, including cellular response to lipopolysaccharide; positive regulation of leukocyte adhesion to vascular endothelial cell; and positive regulation of macromolecule metabolic process. Located in extracellular space. Part of interleukin-6 receptor complex. Implicated in several diseases, including autoimmune disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); glucose metabolism disease (multiple); and periodontal disease (multiple). Biomarker of several diseases, including autoimmune disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); glucose metabolism disease (multiple); and lung disease (multiple).
Mouse Il6 Target Information
- Target Symbol: Il6, interleukin 6
- Alias:
- Il-6
- NCBI_Gene: 16193
Mouse Il6 Predicted Functions
Enables cytokine activity and interleukin-6 receptor binding activity. Involved in several processes, including animal organ development; positive regulation of macromolecule metabolic process; and regulation of peptide hormone secretion. Acts upstream of or within with a positive effect on gene expression. Acts upstream of or within several processes, including cellular response to cytokine stimulus; regulation of gene expression; and salivary gland morphogenesis. Located in external side of plasma membrane and extracellular space. Part of interleukin-6 receptor complex. Is expressed in several structures, including adrenal gland; genitourinary system; gut; liver; and thymus. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); gastrointestinal system cancer (multiple); glucose metabolism disease (multiple); and periodontal disease (multiple). Orthologous to human IL6 (interleukin 6).
Rat Il6 Target Information
- Target Symbol: Il6, interleukin 6
- Alias:
- Ifnb2
- IL-6
- ILg6
- Interleukin 6 (interferon beta 2)
- Interleukin 6 (interferon, beta 2)
- interleukin-6
- NCBI_Gene: 24498
- UniProtKB: P20607
Rat Il6 Predicted Functions
Enables cytokine activity and interleukin-6 receptor binding activity. Involved in several processes, including cellular response to lipid; positive regulation of cell communication; and response to peptide hormone. Located in cytoplasm and extracellular space. Used to study several diseases, including acute necrotizing pancreatitis; disseminated intravascular coagulation; fatty liver disease; hypertension (multiple); and impotence. Biomarker of several diseases, including acute necrotizing pancreatitis; artery disease (multiple); auditory system disease (multiple); iron deficiency anemia; and lung disease (multiple). Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); glucose metabolism disease (multiple); and periodontal disease (multiple). Orthologous to human IL6 (interleukin 6).