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Mouse IGFBP-5 ELISA Kit
$350.00 – $450.00
| SPECIES | Mouse |
|---|---|
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
| Assay Time | 3.5 h |
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
|---|---|
| Assay Time | 3.5 h |
| Platform | ELISA |
| Plate | Detachable 96-well plate |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Assay Principle | This kit utilizes a sandwich enzyme-linked immunosorbent assay (ELISA) technique. Specific anti-mouse IGFBP-5 antibodies are precoated on a high-affinity ELISA plate. Standards, test samples, and biotinylated detection antibodies are added to the wells of the plate and incubated, allowing the IGFBP-5 in the samples to bind to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, HRP-labeled streptavidin is added. After washing again, a chromogenic substrate TMB is added for color development under light protection. The intensity of the color reaction is directly proportional to the concentration of IGFBP-5 in the sample. The reaction is terminated by adding a stop solution, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
