Mouse Dkk-1 ELISA Kit

ELISA Kits

$350.00 – $450.00

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Datasheet

SDS File

TARGETS	DKK1
SPECIES	Mouse
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL
Sensitivity	3.14 pg/mL
Range	93.75 pg/mL – 6000 pg/mL
Assay Time	3.5 h
Recovery	80% – 118%
Average Recovery	1

Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL
Sensitivity	3.14 pg/mL
Range	93.75 pg/mL – 6000 pg/mL
Assay Time	3.5 h
Recovery	80% – 118%
Average Recovery	1
Intra Precision	2.7% – 4.2%
Inter-Precision	2.5% – 4.2%
Platform	ELISA
Plate	Detachable 96-well plate
Size	96T/48T
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Dkk-1 monoclonal antibody Mouse Dkk-1 freeze-dried standard Dkk-1 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse Dkk-1 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, Dkk-1 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of Dkk-1 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Targets

DKK1

DKK1 Target Infomation Overview

Target Symbol: DKK1, dickkopf WNT signaling pathway inhibitor 1
Alias: SK; DKK-1
Alias Names: dickkopf (Xenopus laevis) homolog 1; dickkopf 1 homolog (Xenopus laevis)

DKK1, dickkopf WNT signaling pathway inhibitor 1 Target Infomation by Species

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Human DKK1 Target Information

Target Symbol: DKK1, dickkopf WNT signaling pathway inhibitor 1
Alias:
- dickkopf 1 homolog
- dickkopf related protein-1
- dickkopf-1 like
- dickkopf-like protein 1
- dickkopf-related protein 1
- DKK-1
- hDkk-1
- SK
NCBI_Gene: 22943
UniProtKB: O94907

Human DKK1 Predicted Functions

Enables co-receptor binding activity; low-density lipoprotein particle receptor binding activity; and receptor antagonist activity. Involved in several processes, including regulation of cell fate specification; regulation of gene expression; and regulation of protein phosphorylation. Located in extracellular space and plasma membrane. Implicated in anodontia.

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Mouse Dkk1 Target Information

Target Symbol: Dkk1, dickkopf WNT signaling pathway inhibitor 1
Alias:
- mdkk-1
NCBI_Gene: 13380
- UniprotKB: O54908

Mouse Dkk1 Predicted Functions

Predicted to enable co-receptor binding activity; low-density lipoprotein particle receptor binding activity; and receptor antagonist activity. Involved in several processes, including modulation of age-related behavioral decline; negative regulation of cellular component organization; and positive regulation of macromolecule metabolic process. Acts upstream of or within several processes, including Wnt signaling pathway involved in somitogenesis; embryonic morphogenesis; and negative regulation of signal transduction. Predicted to be located in extracellular region and plasma membrane. Predicted to be active in extracellular space. Is expressed in several structures, including alimentary system; embryo mesenchyme; eye; genitourinary system; and nervous system. Human ortholog(s) of this gene implicated in anodontia. Orthologous to human DKK1 (dickkopf WNT signaling pathway inhibitor 1).

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Rat Dkk1 Target Information

Target Symbol: Dkk1, dickkopf WNT signaling pathway inhibitor 1
Alias:
- dickkopf 1 homolog
- dickkopf 1 homolog (Xenopus laevis)
- dickkopf homolog 1
- dickkopf homolog 1 (Xenopus laevis)
- dickkopf-like protein 1
- dickkopf-related protein 1
- LOC293897
- NEWGENE_1307313
NCBI_Gene: 293897
UniProtKB: D3Z9J1

Rat Dkk1 Predicted Functions

Predicted to enable co-receptor binding activity; low-density lipoprotein particle receptor binding activity; and receptor antagonist activity. Involved in negative regulation of ossification and positive regulation of neuron death. Located in extracellular space. Used to study visual epilepsy. Human ortholog(s) of this gene implicated in anodontia. Orthologous to human DKK1 (dickkopf WNT signaling pathway inhibitor 1).

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