Human TNF-β/Lymphotoxin-α ELISA Kit

$350.00$450.00

TARGETS LTA
SPECIES Human
Sample Type Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume 10 μL
Sensitivity 2.02 pg/mL
Range 62.5 pg/mL – 4000 pg/mL
Assay Time 3.5 h
Recovery 87% – 113%
Average Recovery 1.02
Sample Type Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume 10 μL
Sensitivity 2.02 pg/mL
Range 62.5 pg/mL – 4000 pg/mL
Assay Time 3.5 h
Recovery 87% – 113%
Average Recovery 1.02
Intra Precision 3.0% – 6.2%
Inter-Precision 3.5% – 4.7%
Platform ELISA
Plate Detachable 96-well plate
Size 96T/48T
Storage If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery 4℃ blue ice transportation
Components 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-TNF-β/Lymphotoxin-α monoclonal antibody
Human TNF-β/Lymphotoxin-α freeze-dried standard
TNF-β/Lymphotoxin-α detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay Principle This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human TNF-β antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, TNF-β present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of TNF-β in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

LTA

LTA Target Infomation Overview

  • Target Symbol: LTA, lymphotoxin alpha
  • Gene Groups: Tumor necrosis factor superfamily
  • Alias: TNFSF1; LT
  • Previous Names: TNFB
  • Alias Names: TNF superfamily member 1; lymphotoxin alpha (TNF superfamily, member 1)

LTA, lymphotoxin alpha Target Infomation by Species

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Human LTA Target Information

  • Target Symbol: LTA, lymphotoxin alpha
  • Alias:
    • CDF
    • cholinergic differentiation factor
    • D factor
    • DIA
    • differentiation inhibitory activity
    • differentiation stimulating factor
    • differentiation-inducing factor
    • differentiation-stimulating factor
    • hepatocyte-stimulating factor III
    • HILDA
    • human interleukin in DA cells
    • leukemia inhibitory factor
    • LIF, interleukin 6 family cytokine
    • melanoma-derived LPL inhibitor
    • MLPLI
  • NCBI_Gene: 4049
  • UniProtKB: P01374

Human LTA Predicted Functions

Enables cytokine activity; growth factor activity; and leukemia inhibitory factor receptor binding activity. Involved in several processes, including leukemia inhibitory factor signaling pathway; positive regulation of protein modification process; and regulation of epithelial cell differentiation involved in kidney development. Acts upstream of or within several processes, including positive regulation of MAPK cascade; positive regulation of macrophage differentiation; and positive regulation of protein phosphorylation. Located in cytosol.

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Mouse Lta Target Information

Mouse Lta Predicted Functions

Predicted to enable tumor necrosis factor receptor binding activity. Acts upstream of or within several processes, including lymph node development; positive regulation of immune response; and positive regulation of interferon-gamma production. Predicted to be located in extracellular space. Is expressed in several structures, including extraembryonic component; gut; hemolymphoid system; liver; and reproductive system. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); glucose metabolism disease (multiple); leishmaniasis (multiple); and lung disease (multiple). Orthologous to human LTA (lymphotoxin alpha).

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[su_tab title=”Rat” disabled=”no” anchor=”” url=”” target=”blank” class=”tab-rat”]

Rat Lta Target Information

  • Target Symbol: Lta, lymphotoxin alpha
  • Alias:
    • LOC103690381
    • LT-alpha
    • lymphotoxin A
    • Lymphotoxin alpha (formerly tumor necrosis factor beta)
    • Lymphotoxin alpha (formerly tumor necrosis factor, beta)
    • lymphotoxin alpha (TNF superfamily, member 1)
    • lymphotoxin-alpha
    • lymphotoxin-alpha-like
    • TNF-beta
    • Tnfb
    • tumor necrosis factor beta
    • tumor necrosis factor ligand superfamily member 1
  • NCBI_Gene: 25008
  • UniProtKB: Q06332

Rat Lta Predicted Functions

Predicted to enable cytokine activity and tumor necrosis factor receptor binding activity. Involved in several processes, including negative regulation of fibroblast proliferation; positive regulation of glial cell proliferation; and response to lipopolysaccharide. Located in extracellular space. Biomarker of cystitis. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); glucose metabolism disease (multiple); leishmaniasis (multiple); and lung disease (multiple). Orthologous to human LTA (lymphotoxin alpha).

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LTA Target News

[catlist tags=”lta” template=targetnews thumbnail=yes thumbnail_class=”related-post-media clr” thumbnail_size=833]

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