Human Thrombopoietin/TPO ELISA Kit

$350.00$450.00

TARGETS THPO
SPECIES Human
Sample Type Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume 50 μL
Sensitivity 11.32 pg/mL
Range 62.5 pg/mL – 4000 pg/mL
Assay Time 3.5 h
Recovery 72% – 124%
Average Recovery 0.97
Sample Type Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume 50 μL
Sensitivity 11.32 pg/mL
Range 62.5 pg/mL – 4000 pg/mL
Assay Time 3.5 h
Recovery 72% – 124%
Average Recovery 0.97
Intra Precision 2.6% – 6.4%
Inter-Precision 2.3% – 5.4%
Platform ELISA
Plate Detachable 96-well plate
Size 96T/48T
Storage If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery 4℃ blue ice transportation
Components 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Thrombopoietin/TPO monoclonal antibody
Human Thrombopoietin/TPO freeze-dried standard
Thrombopoietin/TPO detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay Principle This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human TPO antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, TPO present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of TPO in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

THPO

1.7
Identification of Plasma Thrombopoietin Level and Its Significance in Patients with Aplastic Anemia and Myelodysplastic Syndrome
Global Medical Genetics
Systemic Lupus Erythematosus
Transplantation

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