Human Layilin/LAYN ELISA Kit
$350.00 – $450.00
Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
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Sample Volume | 50 μL |
Sensitivity | 4.25 pg/mL |
Range | 31.25 pg/mL – 2000 pg/mL |
Assay Time | 3.5 h |
Recovery | 80% – 111% |
Average Recovery | 0.97 |
Intra Precision | 2.7% – 3.8% |
Inter-Precision | 2.6% – 4.4% |
Platform | ELISA |
Plate | Detachable 96-well plate |
Size | 96T/48T |
Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
Delivery | 4℃ blue ice transportation |
Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Layilin/LAYN monoclonal antibody Human Layilin/LAYN freeze-dried standard Layilin/LAYN detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human LAYN antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and the detection antibody labeled with horseradish peroxidase are added to the wells of the ELISA plate. After incubation, LAYN present in the samples binds to the solid-phase antibody and the detection antibody. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of LAYN in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Targets
LAYN
LAYN Target Infomation Overview
- Target Symbol: LAYN, layilin
- Gene Groups: C-type lectin domain containing
- Alias: FLJ30977; FLJ31092
LAYN, layilin Target Infomation by Species
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Human LAYN Target Information
- Target Symbol: LAYN, layilin
- Alias:
- FLJ30977
- FLJ31092
- NCBI_Gene: 143903
- UniProtKB: Q6UX15
Human LAYN Predicted Functions
Enables hyaluronic acid binding activity. Located in focal adhesion.
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Mouse Layn Target Information
- Target Symbol: Layn, layilin
- Alias:
- E030012M19Rik
- gene model 511, (NCBI)
- Gm511
- LOC244864
- RIKEN cDNA E030012M19 gene
- NCBI_Gene: 244864
Mouse Layn Predicted Functions
Predicted to enable hyaluronic acid binding activity. Predicted to be located in membrane. Predicted to be integral component of membrane. Is expressed in brain and trigeminal ganglion. Orthologous to human LAYN (layilin).
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Rat Layn Target Information
- Target Symbol: Layn, layilin
- Alias:
- LOC500996
- RGD1564444
- similar to layilin
- NCBI_Gene: 500996
Rat Layn Predicted Functions
Predicted to enable hyaluronic acid binding activity. Predicted to be integral component of membrane. Orthologous to human LAYN (layilin).
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LAYN Target News
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