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Human IL-22 High Sensitivity ELISA Kit
$380.00 – $480.00
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
|---|---|
| Sample Volume | Serum, plasma: 20 μL；cell culture supernatant: 100 μL |
| Sensitivity | 0.67 pg/mL |
| Range | 6.25 pg/mL – 400 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 92% – 117% |
| Average Recovery | 1.05 |
| Intra Precision | 4.1% – 4.8% |
| Inter-Precision | 3.8% – 4.7% |
| Platform | ELISA |
| Plate | Detachable 96-well plate |
| Size | 96T/48T |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-22 monoclonal antibody Human IL-22 high sensitivity freeze-dried standard IL-22 detect Antibody Standard Diluent HRP-labeled streptavidin Signal enhancer concentrate Signal enhancer diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IL-22 antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the IL-22 present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing again, a signal enhancer is added and incubated. After washing to remove unbound substances, Streptavidin-HRP is added once more. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-22 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Targets
IL22
IL22 Target Infomation Overview
- Target Symbol: IL22, interleukin 22
- Gene Groups: Interleukins
- Alias: ILTIF; IL-21; zcyto18; IL-TIF; IL-D110; TIFa; TIFIL-23; IL-22; MGC79382; MGC79384
- Alias Names: IL-10-related T-cell-derived inducible factor
IL22, interleukin 22 Target Infomation by Species
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Human IL22 Target Information
- Target Symbol: IL22, interleukin 22
- Alias:
- cytokine Zcyto18
- IL-10-related T-cell-derived inducible factor
- IL-10-related T-cell-derived-inducible factor
- IL-21
- IL-22
- IL-D110
- IL-TIF
- IL21
- ILTIF
- interleukin 21
- interleukin-22
- MGC79382
- MGC79384
- TIFa
- TIFIL-23
- zcyto18
- NCBI_Gene: 50616
- UniProtKB: Q9GZX6
Human IL22 Predicted Functions
Predicted to enable cytokine activity. Predicted to be involved in response to glucocorticoid and signal transduction. Predicted to be located in extracellular region. Biomarker of asthma; rhinitis; and tuberculosis.
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Mouse Il22 Target Information
- Target Symbol: Il22, interleukin 22
- Alias:
- IL-22
- IL-TIF
- Iltif
- interleukin 10-related T cell-derived inducible factor
- NCBI_Gene: 50929
Mouse Il22 Predicted Functions
Predicted to enable cytokine activity. Acts upstream of or within several processes, including negative regulation of inflammatory response; reactive oxygen species metabolic process; and regulation of tyrosine phosphorylation of STAT protein. Predicted to be located in extracellular space. Is expressed in retina and skin. Used to study liposarcoma. Human ortholog(s) of this gene implicated in asthma. Orthologous to human IL22 (interleukin 22).
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Rat Il22 Target Information
- Target Symbol: Il22, interleukin 22
- Alias:
- If2b1
- interferon 2b1
- interleukin-22
- LOC500836
- RGD1561292
- similar to TIF alpha protein
- NCBI_Gene: 500836
Rat Il22 Predicted Functions
Involved in response to glucocorticoid. Located in extracellular space. Used to study asthma and myocarditis. Orthologous to human IL22 (interleukin 22).
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IL22 Target News
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