Human IL-18 ELISA Kit

ELISA Kits

$350.00 – $450.00

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Datasheet

SDS File

TARGETS	IL18
SPECIES	Human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	Serum, plasma: 50 μL；cell culture supernatant: 100 μL
Sensitivity	0.21 pg/mL
Range	156.25 pg/mL – 10000 pg/mL
Assay Time	3.5 h
Recovery	85% – 114%
Average Recovery	1.01

Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	Serum, plasma: 50 μL；cell culture supernatant: 100 μL
Sensitivity	0.21 pg/mL
Range	156.25 pg/mL – 10000 pg/mL
Assay Time	3.5 h
Recovery	85% – 114%
Average Recovery	1.01
Intra Precision	2.4% – 9.4%
Inter-Precision	3.4% – 9.8%
Platform	ELISA
Plate	Detachable 96-well plate
Size	96T/48T
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-18 monoclonal antibody Human IL-18 freeze-dried standard IL-18 detect Antibody Standard Diluent HRP-labeled streptavidin Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×)
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IL-18 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IL-18 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-18 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

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Targets

IL18

IL18 Target Infomation Overview

Target Symbol: IL18, interleukin 18
Gene Groups: Interleukins
Alias: IGIF; IL1F4; IL-1g; IL-18
Alias Names: interferon-gamma-inducing factor; interleukin 18 (interferon-gamma-inducing factor)

IL18, interleukin 18 Target Infomation by Species

HumanMouseRat

Human IL18 Target Information

Target Symbol: IL18, interleukin 18
Alias:
- iboctadekin
- IFN-gamma-inducing factor
- IGIF
- IL-1 gamma
- IL-18
- IL-1g
- IL1F4
- interferon gamma-inducing factor
- interleukin 18 (interferon-gamma-inducing factor)
- interleukin-1 gamma
- interleukin-18
- MGC12320
NCBI_Gene: 3606
UniProtKB: Q14116

Human IL18 Predicted Functions

Enables cytokine activity and interleukin-18 receptor binding activity. Involved in several processes, including positive regulation of cell population proliferation; positive regulation of intracellular signal transduction; and positive regulation of macromolecule metabolic process. Located in extracellular space. Implicated in several diseases, including Kawasaki disease; allergic rhinitis; autoimmune disease (multiple); biliary atresia; and hepatitis B. Biomarker of several diseases, including autoimmune disease (multiple); cholestasis (multiple); liver disease (multiple); lung disease (multiple); and myositis (multiple).

Mouse Il18 Target Information

Target Symbol: Il18, interleukin 18
Alias:
- Igif
- Il-18
- interferon gamma inducing factor
NCBI_Gene: 16173

Mouse Il18 Predicted Functions

Enables cytokine activity. Involved in lipid homeostasis; positive regulation of cell differentiation; and positive regulation of cold-induced thermogenesis. Acts upstream of or within several processes, including interleukin-18-mediated signaling pathway; positive regulation of NIK/NF-kappaB signaling; and positive regulation of interferon-gamma production. Located in extracellular space. Is expressed in several structures, including back skin; dorsal aorta; gut; lymphatic system; and reproductive system. Used to study atopic dermatitis. Human ortholog(s) of this gene implicated in several diseases, including Kawasaki disease; allergic rhinitis; autoimmune disease (multiple); biliary atresia; and hepatitis B. Orthologous to human IL18 (interleukin 18).

Rat Il18 Target Information

Target Symbol: Il18, interleukin 18
Alias:
- IFN-gamma-inducing factor
- IL-1 gamma
- IL-18
- interferon gamma-inducing factor
- interferon-gamma-inducing factor
- interleukin-1 gamma
- interleukin-18
NCBI_Gene: 29197
- UniprotKB: P97636
- UniprotKB: Z4YNI9

Rat Il18 Predicted Functions

Predicted to enable cytokine activity and interleukin-18 receptor binding activity. Involved in several processes, including cellular response to hormone stimulus; positive regulation of cell population proliferation; and positive regulation of cytokine production. Located in apical plasma membrane; cytoplasm; and extracellular space. Used to study brain edema; fatty liver disease; heart disease; status epilepticus; and vasculitis. Biomarker of several diseases, including cardiac arrest; ischemia (multiple); lung disease (multiple); perinatal necrotizing enterocolitis; and urinary tract infection. Human ortholog(s) of this gene implicated in several diseases, including Kawasaki disease; allergic rhinitis; autoimmune disease (multiple); biliary atresia; and hepatitis B. Orthologous to human IL18 (interleukin 18).