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Human HSP27/HSPB1 ELISA Kit
$350.00 – $450.00
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
|---|---|
| Sample Volume | 10 μL |
| Sensitivity | 5.86 pg/mL |
| Range | 31.25 pg/mL – 2000 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 92% – 114% |
| Average Recovery | 1.03 |
| Intra Precision | 3.3% – 6.9% |
| Inter-Precision | 3.2%-4.8% |
| Platform | ELISA |
| Plate | Detachable 96-well plate |
| Size | 96T/48T |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-HSP27/HSPB1 monoclonal antibody Human HSP27/HSPB1 freeze-dried standard Human HSP27/HSPB1 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human HSP27 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and the detection antibody labeled with horseradish peroxidase are added to the wells of the ELISA plate. After incubation, HSP27 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of HSP27 in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Targets
HSPB1
HSPB1 Target Infomation Overview
- Target Symbol: HSPB1, heat shock protein family B (small) member 1
- Gene Groups: Small heat shock proteins
- Alias: HSP27; HSP28; Hs.76067; Hsp25; CMT2F
- Alias Names: heat shock 27kD protein 1; heat shock 27kDa protein 1
HSPB1, heat shock protein family B (small) member 1 Target Infomation by Species
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Human HSPB1 Target Information
- Target Symbol: HSPB1, heat shock protein family B (small) member 1
- Alias:
- 28 kDa heat shock protein
- CMT2F
- DKFZp586P1322
- epididymis secretory protein Li 102
- estrogen-regulated 24 kDa protein
- heat shock 27 kDa protein
- heat shock 27kD protein 1
- heat shock 27kDa protein 1
- heat shock protein beta-1
- HEL-S-102
- HMN2B
- HS.76067
- HSP 27
- Hsp25
- HSP27
- HSP28
- putative heat shock protein family B member 1 isoform
- SRP27
- stress-responsive protein 27
- NCBI_Gene: 3315
- UniProtKB: P04792
Human HSPB1 Predicted Functions
Enables several functions, including protein folding chaperone; protein homodimerization activity; and protein kinase binding activity. Involved in several processes, including anterograde axonal protein transport; positive regulation of angiogenesis; and positive regulation of endothelial cell migration. Located in cytoplasm and nucleus. Implicated in Charcot-Marie-Tooth disease axonal type 2F; distal hereditary motor neuropathy type 2B; pancreatic cancer; and sarcoma. Biomarker of focal segmental glomerulosclerosis and rheumatoid arthritis.
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Mouse Hspb1 Target Information
- Target Symbol: Hspb1, heat shock protein 1
- Alias:
- heat shock protein, 25 kDa
- Hsp25
- NCBI_Gene: 15507
Mouse Hspb1 Predicted Functions
Enables protein kinase C binding activity and protein kinase C inhibitor activity. Involved in negative regulation of protein kinase activity; positive regulation of cytokine production; and regulation of intracellular signal transduction. Acts upstream of or within negative regulation of apoptotic signaling pathway. Located in Z disc; nucleus; and plasma membrane. Is expressed in several structures, including alimentary system; brain; early conceptus; genitourinary system; and heart. Human ortholog(s) of this gene implicated in Charcot-Marie-Tooth disease axonal type 2F; distal hereditary motor neuropathy type 2B; pancreatic cancer; and sarcoma. Orthologous to human HSPB1 (heat shock protein family B (small) member 1).
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Rat Hspb1 Target Information
- Target Symbol: Hspb1, heat shock protein family B (small) member 1
- Alias:
- heat shock 27 kDa protein
- heat shock 27 kDa protein 1
- heat shock 27kDa protein 1
- heat shock protein 1
- heat shock protein B1
- heat shock protein beta-1
- HSP 27
- Hsp25
- Hsp27
- NCBI_Gene: 24471
Rat Hspb1 Predicted Functions
Enables ubiquitin binding activity. Involved in several processes, including cellular response to butyrate; cellular response to interleukin-11; and response to ischemia. Located in several cellular components, including myofibril; perikaryon; and postsynaptic density membrane. Part of proteasome complex. Biomarker of Alzheimer’s disease and transient cerebral ischemia. Human ortholog(s) of this gene implicated in Charcot-Marie-Tooth disease axonal type 2F; distal hereditary motor neuropathy type 2B; pancreatic cancer; and sarcoma. Orthologous to human HSPB1 (heat shock protein family B (small) member 1).
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HSPB1 Target News
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