Human GM-CSF ELISA Kit

ELISA Kits

$350.00 – $450.00

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Datasheet

SDS File

TARGETS	CSF2
SPECIES	Human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	20 μL
Sensitivity	4.76 pg/mL
Range	1.56 pg/mL – 100 pg/mL
Assay Time	3.5 h
Recovery	97% – 110%
Average Recovery	1.04

Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	20 μL
Sensitivity	4.76 pg/mL
Range	1.56 pg/mL – 100 pg/mL
Assay Time	3.5 h
Recovery	97% – 110%
Average Recovery	1.04
Intra Precision	2.5% – 2.9%
Inter-Precision	3.7% – 4.9%
Platform	ELISA
Plate	Detachable 96-well plate
Size	96T/48T
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-GM-CSF monoclonal antibody Human GM-CSF freeze-dried standard GM-CSF detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human GM-CSF antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, GM-CSF present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of GM-CSF in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Targets

CSF2

CSF2 Target Infomation Overview

Target Symbol: CSF2, colony stimulating factor 2
Alias: GM-CSF; GMCSF
Alias Names: sargramostim; molgramostim; granulocyte-macrophage colony stimulating factor; colony stimulating factor 2 (granulocyte-macrophage)

CSF2, colony stimulating factor 2 Target Infomation by Species

HumanMouseRat

Human CSF2 Target Information

Target Symbol: CSF2, colony stimulating factor 2
Alias:
- colony stimulating factor 2 (granulocyte-macrophage)
- colony-stimulating factor
- CSF
- GMCSF
- granulocyte macrophage-colony stimulating factor
- granulocyte-macrophage colony stimulating factor
- granulocyte-macrophage colony-stimulating factor
- MGC131935
- MGC138897
- molgramostim
- molgramostin
- sargramostim
NCBI_Gene: 1437
UniProtKB: P04141

Human CSF2 Predicted Functions

Enables cytokine activity. Involved in several processes, including cellular response to granulocyte macrophage colony-stimulating factor stimulus; myeloid leukocyte differentiation; and positive regulation of macromolecule metabolic process. Located in extracellular space and intracellular membrane-bounded organelle. Implicated in acute myeloid leukemia; juvenile myelomonocytic leukemia; neutropenia; pulmonary alveolar proteinosis; and visceral leishmaniasis. Biomarker of COVID-19; asthma; chronic obstructive pulmonary disease; and temporal arteritis.

Mouse Csf2 Target Information

Target Symbol: Csf2, colony stimulating factor 2 (granulocyte-macrophage)
Alias:
- colony stimulating factor, granulocyte macrophage
- Csfgm
- Gm-CSf
- GMCSF
- MGI-IGM
NCBI_Gene: 12981

Mouse Csf2 Predicted Functions

Enables cytokine activity. Acts upstream of or within several processes, including embryonic placenta development; myeloid leukocyte differentiation; and positive regulation of cell population proliferation. Located in extracellular space. Is expressed in several structures, including genitourinary system; hemolymphoid system; labyrinthine zone; liver; and lung. Used to study pulmonary alveolar proteinosis. Human ortholog(s) of this gene implicated in acute myeloid leukemia; juvenile myelomonocytic leukemia; neutropenia; pulmonary alveolar proteinosis; and visceral leishmaniasis. Orthologous to human CSF2 (colony stimulating factor 2).

Rat Csf2 Target Information

Target Symbol: Csf2, colony stimulating factor 2
Alias:
- colony stimulating factor 2 (granulocyte-macrophage)
- colony-stimulating factor
- CSF
- Gm-csf
- Gmcsf
- granulocyte-macrophage colony-stimulating factor
NCBI_Gene: 116630
UniProtKB: P48750

Rat Csf2 Predicted Functions

Enables cytokine activity. Involved in several processes, including cellular response to lipopolysaccharide; macrophage activation; and response to silicon dioxide. Located in extracellular space. Used to study Parkinsonism and myocardial infarction. Biomarker of abdominal aortic aneurysm; interstitial cystitis; and lung disease (multiple). Human ortholog(s) of this gene implicated in acute myeloid leukemia; juvenile myelomonocytic leukemia; neutropenia; pulmonary alveolar proteinosis; and visceral leishmaniasis. Orthologous to human CSF2 (colony stimulating factor 2).