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Human Angiopoietin-1/ANG-1 ELISA Kit
$350.00 – $450.00
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
|---|---|
| Sample Volume | 50 μL |
| Sensitivity | 1.30 pg/mL |
| Range | 31.25 pg/mL – 2000 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 80% – 110% |
| Average Recovery | 0.94 |
| Intra Precision | 3.3% – 5.4% |
| Inter-Precision | 5.0% – 8.2% |
| Platform | ELISA |
| Plate | Detachable 96-well plate |
| Size | 96T/48T |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Angipoietin-1/ANG-1 monoclonal antibody Human Angipoietin-1/ANG-1 freeze-dried standard Angipoietin-1/ANG-1 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human ANG-1 antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the ANG-1 present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of ANG-1 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Targets
ANGPT1
ANGPT1 Target Infomation Overview
- Target Symbol: ANGPT1, angiopoietin 1
- Gene Groups: Receptor ligands; Fibrinogen C domain containing
- Alias: KIAA0003; Ang1
ANGPT1, angiopoietin 1 Target Infomation by Species
HumanMouseRat
Human ANGPT1 Target Information
- Target Symbol: ANGPT1, angiopoietin 1
- Alias:
- AGP1
- AGPT
- ANG-1
- ANG1
- angiopoietin-1
- HAE5
- NCBI_Gene: 284
- UniProtKB: Q15389
Human ANGPT1 Predicted Functions
Enables receptor tyrosine kinase binding activity. Involved in several processes, including positive regulation of intracellular signal transduction; positive regulation of macromolecule metabolic process; and regulation of vascular permeability. Located in several cellular components, including extracellular space; membrane raft; and microvillus. Implicated in brain ischemia; diabetic retinopathy; and hypertension. Biomarker of carcinoma (multiple); hypertension; and ovarian cancer.
Mouse Angpt1 Target Information
- Target Symbol: Angpt1, angiopoietin 1
- Alias:
- 1110046O21Rik
- Ang-1
- ang1
- Angiopoietin-1
- RIKEN cDNA 1110046O21 gene
- NCBI_Gene: 11600
Mouse Angpt1 Predicted Functions
Enables signaling receptor binding activity. Acts upstream of or within several processes, including blood vessel development; regulation of protein phosphorylation; and regulation of signal transduction. Predicted to be located in membrane raft; microvillus; and plasma membrane. Predicted to be active in collagen-containing extracellular matrix and extracellular space. Is expressed in several structures, including cardiovascular system; liver; lung; metanephros; and yolk sac. Human ortholog(s) of this gene implicated in brain ischemia; diabetic retinopathy; and hypertension. Orthologous to human ANGPT1 (angiopoietin 1).
Rat Angpt1 Target Information
- Target Symbol: Angpt1, angiopoietin 1
- Alias:
- Agpt
- Agpt1
- Ang-1
- angiopoietin
- angiopoietin-1
- LOC360422
- NCBI_Gene: 89807
Rat Angpt1 Predicted Functions
Enables identical protein binding activity. Involved in several processes, including animal organ development; response to estrogen; and response to vitamin B3. Located in extracellular space. Biomarker of gastric ulcer; in situ carcinoma; myocardial infarction; nephrosis; and pulmonary hypertension. Human ortholog(s) of this gene implicated in brain ischemia; diabetic retinopathy; and hypertension. Orthologous to human ANGPT1 (angiopoietin 1).
