Human ACE ELISA Kit

ELISA Kits

$350.00 – $450.00

Download:

Datasheet

SDS File

TARGETS	ACE
SPECIES	Human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL(diluent)
Sensitivity	8.34 pg/mL
Range	125 pg/mL – 8000 pg/mL
Assay Time	3.5 h
Recovery	90% – 110%
Average Recovery	1.03

Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL(diluent)
Sensitivity	8.34 pg/mL
Range	125 pg/mL – 8000 pg/mL
Assay Time	3.5 h
Recovery	90% – 110%
Average Recovery	1.03
Intra Precision	2.6% – 3.5%
Inter-Precision	2.7%-4.2%
Platform	ELISA
Plate	Detachable 96-well plate
Size	96T/48T
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-ACE monoclonal antibody Human ACE freeze-dried standard Human ACE detect Antibody Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human ACE antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, ACE present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of ACE in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Targets

ACE

ACE Target Infomation Overview

Target Symbol: ACE, angiotensin I converting enzyme
Gene Groups: CD molecules
Alias: ACE1; CD143
Previous Names: DCP1
Alias Names: peptidyl-dipeptidase A; angiotensin I converting enzyme (peptidyl-dipeptidase A) 1

ACE, angiotensin I converting enzyme Target Infomation by Species

[su_tabs][su_tab title=”Human” disabled=”no” anchor=”” url=”” target=”blank” class=”tab-human”]

Human ACE Target Information

Target Symbol: ACE, angiotensin I converting enzyme
Alias:
- ACE1
- angiotensin 1 converting enzyme 1
- angiotensin converting enzyme, somatic isoform
- angiotensin I converting enzyme (peptidyl-dipeptidase A) 1
- angiotensin I converting enzyme peptidyl-dipeptidase A 1 transcript
- angiotensin-converting enzyme
- carboxycathepsin
- CD143
- CD143 antigen
- DCP
- DCP1
- dipeptidyl carboxypeptidase 1
- dipeptidyl carboxypeptidase I
- ICH
- kininase II
- MGC26566
- MVCD3
- peptidase P
- testicular ECA
NCBI_Gene: 1636
UniProtKB: P12821

Human ACE Predicted Functions

Enables several functions, including chloride ion binding activity; peptidase activity; and protein kinase binding activity. Involved in several processes, including arachidonic acid secretion; peptide metabolic process; and regulation of angiotensin metabolic process. Located in several cellular components, including external side of plasma membrane; extracellular exosome; and lysosome. Implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); gastrointestinal system cancer (multiple); heart valve disease (multiple); and lung disease (multiple). Biomarker of several diseases, including chronic myeloid leukemia; intermediate coronary syndrome; lung disease (multiple); pancreatic ductal carcinoma; and renal fibrosis.

[/su_tab]
[su_tab title=”Mouse” disabled=”no” anchor=”” url=”” target=”blank” class=”tab-mouse”]

Mouse Ace Target Information

Target Symbol: Ace, angiotensin I converting enzyme (peptidyl-dipeptidase A) 1
Alias:
- AW208573
- CD143
- expressed sequence AW208573
NCBI_Gene: 11421

Mouse Ace Predicted Functions

Enables metallopeptidase activity and peptidyl-dipeptidase activity. Involved in several processes, including cell proliferation in bone marrow; positive regulation of protein modification process; and regulation of gene expression. Acts upstream of or within several processes, including heart contraction; neutrophil mediated immunity; and positive regulation of systemic arterial blood pressure. Located in cytoplasm and plasma membrane. Is expressed in several structures, including alimentary system; brain; extraembryonic component; genitourinary system; and integumental system. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); gastrointestinal system cancer (multiple); heart valve disease (multiple); and lung disease (multiple). Orthologous to human ACE (angiotensin I converting enzyme).

[/su_tab]
[su_tab title=”Rat” disabled=”no” anchor=”” url=”” target=”blank” class=”tab-rat”]

Rat Ace Target Information

Target Symbol: Ace, angiotensin I converting enzyme
Alias:
- angiotensin 1 converting enzyme
- angiotensin 1 converting enzyme 1
- angiotensin converting enzyme
- angiotensin I converting enzyme (peptidyl-dipeptidase A) 1
- angiotensin I converting enzyme 1
- angiotensin I-converting enzyme (Dipeptidyl carboxypeptidase 1)
- angiotensin-converting enzyme
- angiotensin-converting enzyme-like
- CD143
- Dcp1
- Dipeptidyl carboxypeptidase 1
- Dipeptidyl carboxypeptidase 1 (Angiotensin I-converting enzyme)
- dipeptidyl carboxypeptidase I
- kininase II
- LOC102556346
- StsRR92
NCBI_Gene: 24310

Rat Ace Predicted Functions

Enables heterocyclic compound binding activity; metallopeptidase activity; and peptidyl-dipeptidase activity. Involved in several processes, including animal organ development; negative regulation of transport; and response to corticosteroid. Located in several cellular components, including basal plasma membrane; brush border membrane; and sperm midpiece. Used to study several diseases, including artery disease (multiple); glomerulonephritis (multiple); kidney failure (multiple); lung disease (multiple); and proteinuria (multiple). Biomarker of several diseases, including artery disease (multiple); congenital diaphragmatic hernia; endomyocardial fibrosis; liver cirrhosis (multiple); and lung disease (multiple). Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); gastrointestinal system cancer (multiple); heart valve disease (multiple); and lung disease (multiple). Orthologous to human ACE (angiotensin I converting enzyme).

[/su_tab][/su_tabs]