Product Category
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Human 4-1BB/TNFRSF9/CD137 ELISA Kit
$350.00 – $450.00
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
|---|---|
| Sample Volume | 10 μL |
| Sensitivity | 1.58 pg/mL |
| Range | 4.69 pg/mL – 300 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 85% – 117% |
| Average Recovery | 1.02 |
| Intra Precision | 4.7% – 5.0% |
| Inter-Precision | 3.2% – 6.6% |
| Platform | ELISA |
| Plate | Detachable 96-well plate |
| Size | 96T/48T |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-4-1BB/TNFRSF9/CD137 monoclonal antibody Human 4-1BB/TNFRSF9/CD137 freeze-dried standard 4-1BB/TNFRSF9/CD137 detect Antibody Standard Diluent HRP-labeled streptavidin Signal enhancer concentrate Signal enhancer diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human 4-1BB antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, 4-1BB present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of 4-1BB in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Targets
TNFRSF9
TNFRSF9 Target Infomation Overview
- Target Symbol: TNFRSF9, TNF receptor superfamily member 9
- Gene Groups: CD molecules; Tumor necrosis factor receptor superfamily
- Alias: CD137; 4-1BB
- Previous Names: ILA
- Alias Names: tumor necrosis factor receptor superfamily, member 9
TNFRSF9, TNF receptor superfamily member 9 Target Infomation by Species
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Human TNFRSF9 Target Information
- Target Symbol: TNFRSF9, TNF receptor superfamily member 9
- Alias:
- 4-1BB
- 4-1BB ligand receptor
- CD137
- CD137 antigen
- CDw137
- FLJ43501
- homolog of mouse 4-1BB
- ILA
- induced by lymphocyte activation (ILA)
- interleukin-activated receptor, homolog of mouse Ly63
- MGC2172
- receptor protein 4-1BB
- T cell antigen ILA
- T-cell antigen 4-1BB homolog
- T-cell antigen ILA
- tumor necrosis factor receptor superfamily member 9
- tumor necrosis factor receptor superfamily, member 9
- NCBI_Gene: 3604
- UniProtKB: Q07011
Human TNFRSF9 Predicted Functions
Predicted to enable cytokine binding activity; identical protein binding activity; and signaling receptor activity. Predicted to be involved in regulation of cell population proliferation. Predicted to act upstream of or within negative regulation of interleukin-10 production and negative regulation of interleukin-12 production. Predicted to be located in extracellular space. Predicted to be active in external side of plasma membrane.
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[su_tab title=”Mouse” disabled=”no” anchor=”” url=”” target=”blank” class=”tab-mouse”]
Mouse Tnfrsf9 Target Information
- Target Symbol: Tnfrsf9, tumor necrosis factor receptor superfamily, member 9
- Alias:
- 4-1BB
- A930040I11Rik
- AA408498
- AI325004
- CD antigen 137
- Cd137
- CDw137
- expressed sequence AA408498
- expressed sequence AI325004
- ILA
- Ly63
- lymphocyte antigen 63
- RIKEN cDNA A930040I11 gene
- NCBI_Gene: 21942
Mouse Tnfrsf9 Predicted Functions
Enables cytokine binding activity; identical protein binding activity; and signaling receptor activity. Acts upstream of or within negative regulation of interleukin-10 production; negative regulation of interleukin-12 production; and regulation of cell population proliferation. Located in external side of plasma membrane and extracellular space. Is expressed in renal vasculature. Orthologous to human TNFRSF9 (TNF receptor superfamily member 9).
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Rat Tnfrsf9 Target Information
- Target Symbol: Tnfrsf9, TNF receptor superfamily member 9
- Alias:
- 4-1BB
- LOC500590
- MGC114552
- similar to T-cell antigen 4-1BB precursor – mouse
- tumor necrosis factor receptor superfamily member 9
- tumor necrosis factor receptor superfamily, member 9
- NCBI_Gene: 500590
- UniProtKB: Q4V895
Rat Tnfrsf9 Predicted Functions
Predicted to enable cytokine binding activity; identical protein binding activity; and signaling receptor activity. Predicted to be involved in regulation of cell population proliferation. Predicted to act upstream of or within negative regulation of interleukin-10 production and negative regulation of interleukin-12 production. Predicted to be located in extracellular space and plasma membrane. Predicted to be active in external side of plasma membrane. Orthologous to human TNFRSF9 (TNF receptor superfamily member 9).
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TNFRSF9 Target News
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