EasyGo!™ Human VEGF One-Step ELISA Kit

EasyGo! One-Step ELISA Kits

$400.00 – $500.00

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Datasheet

SDS File

TARGETS	VEGFA
SPECIES	Human
Sample Type	Serum, plasma, DMEM cell culture supernatant
Sample Volume	Serum or plasma: 20μL; DMEM cell culture supernatant: 100μL
Sensitivity	3.03 pg/mL
Range	31.25 pg/mL – 2000 pg/mL
Assay Time	1.5 h
Recovery	85% – 112%
Average Recovery	0.94

Size

48T

96T

Clear

Sample Type	Serum, plasma, DMEM cell culture supernatant
Sample Volume	Serum or plasma: 20μL; DMEM cell culture supernatant: 100μL
Sensitivity	3.03 pg/mL
Range	31.25 pg/mL – 2000 pg/mL
Assay Time	1.5 h
Recovery	85% – 112%
Average Recovery	0.94
Intra Precision	4.1% – 6.4%
Inter-Precision	2.1% – 2.6%
Platform	ELISA
Plate	Detachable 96-well plate
Size	96T/48T
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-VEGF monoclonal antibody Human VEGF freeze-dried standard VEGF detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This assay employs the quantitative sandwich enzyme immunoassay technique. Amonoclonal antibody specific for human VEGF has been immobilized onto microwells, and two pellets of the biotin-linked detect antibody specific for VEGF (light yellow) and streptavidin-HRP (purple) are pre-placed in the microwells, sealed by the adhesive film. Standard or samples are pipetted intothe wells, then VEGF present is bound by the immobilized antibody and detect antibody, of whichthe latter is conjugated with streptavidin-HRP in the incubation. After washing, substrate solutionreacts with HRP and color develops in proportion to the amount of VEGF bound by the immobilized antibody. The color development is stopped and the intensity of the color is measuredby microplate reader.

Targets

VEGFA

VEGFA Target Infomation Overview

Target Symbol: VEGFA, vascular endothelial growth factor A
Gene Groups: VEGF family
Alias: VEGF-A; VPF
Previous Names: VEGF
Alias Names: vascular endothelial growth factor

VEGFA, vascular endothelial growth factor A Target Infomation by Species

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Human VEGFA Target Information

Target Symbol: VEGFA, vascular endothelial growth factor A
Alias:
- MGC70609
- MVCD1
- vascular endothelial growth factor
- vascular endothelial growth factor A121
- vascular endothelial growth factor A165
- vascular permeability factor
- VEGF
- VEGF-A
- VPF
NCBI_Gene: 7422
UniProtKB: P15692

Human VEGFA Predicted Functions

Enables several functions, including fibronectin binding activity; heparin binding activity; and signaling receptor binding activity. Involved in several processes, including positive regulation of cell migration; positive regulation of intracellular signal transduction; and positive regulation of macromolecule metabolic process. Acts upstream of or within several processes, including branching involved in blood vessel morphogenesis; positive regulation of endothelial cell proliferation; and regulation of nitric oxide mediated signal transduction. Located in several cellular components, including adherens junction; cell surface; and secretory granule. Implicated in several diseases, including autoimmune disease (multiple); gastrointestinal system cancer (multiple); hematologic cancer (multiple); hypertension (multiple); and reproductive organ cancer (multiple). Biomarker of several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); inflammatory bowel disease (multiple); and respiratory system cancer (multiple).

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Mouse Vegfa Target Information

Target Symbol: Vegfa, vascular endothelial growth factor A
Alias:
- vascular endothelial growth factor
- Vegf
- VEGF-A
- VEGF120
- VEGF164
- VEGF188
- VPF
NCBI_Gene: 22339

Mouse Vegfa Predicted Functions

Enables extracellular matrix binding activity; heparin binding activity; and signaling receptor binding activity. Involved in several processes, including generation of neurons; regulation of signal transduction; and vasculature development. Acts upstream of or within several processes, including animal organ development; positive regulation of cell population proliferation; and vasculature development. Located in cytoplasm; extracellular space; and nucleus. Is expressed in several structures, including alimentary system; brain; egg cylinder; genitourinary system; and heart. Used to study DiGeorge syndrome; amyotrophic lateral sclerosis type 1; newborn respiratory distress syndrome; and psoriasis. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); gastrointestinal system cancer (multiple); hematologic cancer (multiple); and reproductive organ cancer (multiple). Orthologous to human VEGFA (vascular endothelial growth factor A).

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Rat Vegfa Target Information

Target Symbol: Vegfa, vascular endothelial growth factor A
Alias:
- vascular endothelial growth factor
- vascular permeability factor
- Vegf
- VEGF-A
- VEGF111
- VEGF164
- VPF
NCBI_Gene: 83785

Rat Vegfa Predicted Functions

Enables several functions, including identical protein binding activity; receptor ligand activity; and vascular endothelial growth factor receptor 2 binding activity. Involved in several processes, including negative regulation of cell death; response to steroid hormone; and response to vitamin. Located in basement membrane; extracellular space; and vesicle. Used to study several diseases, including brain edema; limb ischemia; middle cerebral artery infarction; ovarian hyperstimulation syndrome; and proliferative diabetic retinopathy. Biomarker of several diseases, including adhesions of uterus; attention deficit hyperactivity disorder; brain edema; liver disease (multiple); and retinal vascular disease (multiple). Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); gastrointestinal system cancer (multiple); hematologic cancer (multiple); hypertension (multiple); and reproductive organ cancer (multiple). Orthologous to human VEGFA (vascular endothelial growth factor A).

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