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EasyGo!™ Human IgG One-Step ELISA Kit
$447.00 – $569.00
| Sample Type | Serum, plasma |
|---|---|
| Sample Volume | 5μL |
| Sensitivity | 0.04 pg/mL |
| Range | 1.56 pg/mL – 100 pg/mL |
| Assay Time | 1.5 h |
| Recovery | 77% – 122% |
| Average Recovery | 91% |
| Intra Precision | 8.1% – 9.0% |
| Inter-Precision | 5.8% – 7.8% |
| Platform | ELISA |
| Plate | Detachable 96-well plate |
| Size | 96T/48T |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | IgG Microplate IgG Standard Assay Buffer (10×) Substrate Stop Solution Washing Buffer (20×) |
| Assay Principle | This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Human IgG has been immobilized onto microwells, and one pellet of the HRP-linked detect antibody specific for IgG (light yellow) is pre-placed in the microwells, sealed by the adhesive film. Standard or samples are pipetted into the wells, then IgG present is bound by the immobilized antibody and detect antibody. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of IgG bound by the immobilized antibody. The color development is stopped and the intensity of the color is measured by microplate reader. |
